columns used in HPLC Things To Know Before You Buy
The focus of polynuclear aromatic hydrocarbons (PAH) in soil is decided by first extracting the PAHs with methylene chloride. The extract is diluted, if needed, plus the PAHs separated by HPLC using a UV/Vis or fluorescence detector. Calibration is obtained employing a number of external requirements. In a standard analysis a two.013-g sample of dr